Chronic stress in rats induces bladder hyperalgesia

Latest research and happenings
Post Reply
User avatar
webslave
Maintenance
Maintenance
Posts: 11390
Joined: Wed Oct 30, 2002 3:18 pm
Location: Please give your location so we can help better
Contact:

Chronic stress in rats induces bladder hyperalgesia

Post by webslave »

This proves that in rats high stress levels can cause a long-lived bladder hyperalgesia
Physiol Behav. 2014 Nov 20;139C:541-548. doi: 10.1016/j.physbeh.2014.11.045. [Epub ahead of print]
Chronic psychological stress in high-anxiety rats induces sustained bladder hyperalgesia.
Lee UJ1, Ackerman AL2, Wu A2, Zhang R2, Leung J2, Bradesi S3, Mayer EA3, Rodríguez LV4.

OBJECTIVE: To evaluate whether anxiety-prone rats exposed to chronic water avoidance stress (WAS) develop visceral bladder hyperalgesia in addition to increased voiding frequency and anxiety-related behaviors.

MATERIALS AND METHODS: Female Wistar-Kyoto (WKY) rats were exposed to chronic (10-day) WAS or sham paradigms. Referred hyperalgesia and tactile allodynia were tested using von Frey filaments applied to the suprapubic region and plantar region of the hindpaw, respectively. To confirm that suprapubic nociception represented referred visceral bladder hyperalgesia, we recorded abdominal visceromotor responses (VMR) to slow (100μl/min) and fast (1cc/sec) bladder filling with room temperature or ice-cold saline. We assessed the development of hyperalgesia over the 10-day WAS protocol and the durability of increased pain sensations over time.

RESULTS: Animals exposed to chronic WAS had significantly lower hindpaw withdrawal thresholds post-stress and significant differences in referred hyperalgesia. Rats exposed to chronic WAS demonstrated an increased pain response to suprapubic stimulation and decreased response threshold to mechanical hindpaw stimulation by day 8 of the stress protocol, which persisted for more than one month. Animals exposed to chronic WAS showed increased VMR to fast filling and ice water testing in comparison to sham animals. Cystometry under anesthesia did not show increases in the frequency of non-voiding contractions.

CONCLUSION: Chronic WAS induces sustained bladder hyperalgesia, lasting over a month after exposure to stress. The urinary frequency demonstrated previously in anxiety-prone rats exposed to chronic WAS seems to be associated with bladder hyperalgesia, suggesting that this is a potential model for future studies of bladder hypersensitivity syndromes such as interstitial cystitis/painful bladder syndrome (IC/PBS).

PMID: 25449389
HAS THIS SITE HELPED YOU?
Say Thanks! by making a small donation
PayPal link at end of page ↓
Please fill out your signature, click here for how to do it
Post Reply